Kev faib tawm ntawm coenzymes uas tsis meej pem

Coenzymes, cofactors, thiab ua kom lub zog
Raws li cov cai ntawm cov kev xav tau ntawm cov kev ua kom zoo rau enzyme catalyzed kev tawm tsam yuav tsum tau ntxiv rau enzyme assay perceple. Cov chaw ua haujlwm xa mus rau cov khoom siv protein uas yuav tsum muaj rau cov haujlwm enzyme, suav nrog coenzymes, cofactors, thiab hlau cov kev ua kom ua. Cov cofactors ze ze rau cov enzymes tau hu ua cofactors; Enzyme protein yam tsis muaj cofactors yog hu ua apozymes, uas tsis muaj catalalytic kev ua ub no thiab xav tau ntxiv ntawm cov cafactors txaus los ua Holozymes. Tom qab ua kom muaj zog nrog Cofactor rau ib lub sijhawm, cov haujlwm enzyme yuav rov qab. Yog li ntawd, nws yog feem ntau yuav tsum tau ua ntej muab cov choulorate hauv cov qauv thiab reagent. Tus nqi ntawm ib qho kev siv hluav taws xob siv yog feem ntau kuj yog me me.
Coenzzymes, uas khi ntau heev rau enzyme protein thiab tuaj yeem sib cais yooj yim los ntawm kev lim ntshav thiab lwm txoj kev, yog hu ua coenzymes. Txawm hais tias Coenzymes sib txawv ntawm enzyme substrates, lawv ua tus zoo ib yam li substrates, khi, sib cais, thiab kev caij tsheb kauj vab, thiab caij tsheb kauj vab nrog enzymes thaum lub sij hawm enzymatic. Kev txiav txim ntawm coenzyme noj tuaj yeem ua los ntawm kev kho lawv raws li subtrates. Piv txwv li, coenzzymes nyob rau hauv lactate dehydrogenase tau muab xam raws li tus lej subtrate sib npaug.
Cov tshuaj siv tshuaj ntawm ib qho activator yog ib qho hlau ion, uas tuaj yeem yog qhov chaw nquag ntawm cov enzyme lossis qhib nws cov kev ua ub no los ntawm lwm yam kev ua. Raws li cov ua haujlwm, hlau ions muaj ntau qhov cuam tshuam rau ntawm kinetics ntawm enzymatic cov tshuaj tiv thaiv. Feem ntau cov hlau ions xws li mg2+, thiab lwm yam}}}}}}}}}}}}}}}}}}}} Hlau ions feem ntau ua rau kev tawm tsam kev tawm tsam lossis kev cuam tshuam. Edta feem ntau ntxiv rau enzyme assay systems rau chelate ib co tsis tseem yog tseem ceeb ions. Ib qho kev tsim nyog ntawm cov hlau ions yog qhov tsim nyog, raws li ntau as suav feem ntau inhibit tus nqi ntawm enzyme tshua. Vim yog qhov tseeb tias lub kinetics ntawm cov kev ua kom sib txawv ntawm cov enzymes ntau zaus, qhov no tuaj yeem piav qhia cov kev sib piv sib txawv, ua rau muaj kev tawm tsam kev ntsuas ua tau zoo. Cov kev ua kom muaj zog ntawm n- acetylcysteine ​​ntawm Creation Kinase yog zoo sib xws. Qhov ntau npaum ntawm cov activator feem ntau yog txiav txim los ntawm kev sim ua dua.